Automated online control of bacteria fermentation to detect the cell growth with an optical density measuring sensor
Diego Bochoeyer Sr.¹, Maria Florencia Perez Lippi², Maximiliano Dxalessio³, Gustavo Stasiejko⁴, Diego Petruzzi¹ and Gabriel Gutkind¹, (1)Fermentation, Novozymes BioAg, Buenos Aires, Argentina, (2)Marketing, Endress + Hausser, Buenos Aires, Argentina, (3)Development & Process Support, Novozymes BioAg, Milwaukee, MN, (4)Sales, Endress + Hausser, Buenos Aires, Argentina

Systems biology approaches to develop a robust process platform for therapeutic protein production by glycoengineered Pichia pastoris
Sehoon Kim¹, Robert Davidson¹, Victoria Copeland¹, Marc d'Anjou¹, Adam Nylen¹, Muralidhar Mallem¹, Ishaan Shandil¹, Seemab Shaikh¹, Khanita Karaveg¹, Brian Mickus² and I-Ming Wang², (1)GlycoFi/ Merck, Lebanon, NH, (2)Merck, West Point, PA

PRODUCTION AND KINETICS OF BIOETHANOL FROM SAWDUST HYDROLYSATE USING MUTANT STRAIN OF Saccharomyces cerevisiae.
Bharathiraja Balasubramaniyan, Praveen kumar Ramanujam, Prukinth Raj Mascow and Palani Subramaniyam, Biotechnology, Arunai Engineering College, Tiruvannamalai, India

Ethanol production from pretreated eucalyptus wood chips by simultaneous saccharification and fermentation
Hibiki Matsushita¹, Noriko Yasutani¹, Kazunori Habu¹, Hirokazu Kikuta¹, Hideshi Yanase², Masakazu Daidai³, Hisayuki Sego⁴, Yoshiki Yasue⁴ and Takashi Watanabe³, (1)Biotechnology & Afforestation Business Division, Toyota Motor Corporation, Miyoshi-shi, Aichi, Japan, (2)Department of Chemistry and Biotechnology, Graduate School of Engineering, Tottori University, Tottori, Japan, (3)Research Institute for Sustainable Humanosphere (RISH), Uji, Japan, (4)Japan Chemical Engineering & Machinery Co., Ltd., Osaka, Japan

A systems biology approach for the production of itaconic acid in filamentous fungi
An Li, Nicole van Luijk, Mariet van der Werf and Peter J. Punt, Microbiology & Systems Biology, TNO, Zeist, Netherlands

Escherichia coli Growth and Production Response to Various Trace Metal Levels
Sucheta G. Vajrala, Mark Berge and Kathrine Allikian, Process Cell Culture and Fermentation, MedImmune, Gaithersburg, MD

Fermentation characteristics of an engineered Saccharomyces cerevisiae on cocoa pod hemicellulosic hydrolysate
Anthony Abiodun Onilude, Richard Osaretin Igbinadolor and Sherifah Monilola Wakil, Microbiology, University of Ibadan, Ibadan, Nigeria

Microscale Fermentation Makes Isotope Labeled Metabolic Flux Analysis an Economical High-Throughput Process
Matthew F. Conway, Ashish Misra, Joseph Johnnie and Ganesh Sriram, Chemical and Biomolecular Engineering, University of Maryland, College Park, MD

Improving Aspergillus niger as cell factory for heterologous protein production through transcription factor modulation
Lars Poulsen, Jens Christian Froeslev Nielsen, Anna Eilasson Lantz and Jette Thykaer, Department of Systems Biology, DTU - Technical University of Denmark, Kgs. Lyngby, Denmark

Bispecific Antibody production in E. coli: Half the Story
James Giulianotti, Genentech, Inc., South San Francisco, CA

Towards an integrated process for the biocatalytic production of 2,5-furan dicarboxylic acid (FDCA)
Tom D. Elink Schuurman and Harald J. Ruijssenaars, BIRD Engineering B.V., Schiedam, Netherlands

Mining for active strains in a large strain library
Paolo Monciardini¹, Margherita Sosio², Stefania Serina¹, Sonia Maffioli², Eleonora Gaspari³, Cristina Brunati³, Paola Guglierame³, Ambrogina Guindani¹ and Stefano Donadio², (1)KtedoGen, Milano, Italy, (2)NAICONS, Milano, Italy, (3)NeED Pharma, Milano, Italy

Characterization of a High-throughput Bioreactor for Cell Line Selection and Process Development
Torben Bruck¹, Tiffany D. Rau², David Loer³, Lacey Douthit³, Savanah Howe³ and Lawrence Chew¹, (1)Fermentation Development, Pfenex Inc, San Diego, CA, (2)Life Science, Pall Corporation, Port Washington, NY, (3)Intern, Pfenex Inc, San Diego, CA

Near and Future Routes to Cellulosic Bio-Conversion Processes; A Technoeconomic Assessment of the Path Forward
Iva J. Tews, Susanne B. Jones and Pimphan Meyer, Energy and Environment Directorate, Pacific Northwest National Lab, Richland, WA

Upstream optimization of glycoengineered Pichia pastoris expressing a highly sialylated therapeutic glycoprotein
Muralidhar Reddy Mallem, Adam C Nylen, Marc d'Anjou, Sujatha Gomathinayagam, Eric Brown, Stephen Hamilton, John Bukowski, Daniel Hopkins, Irina Burnina, Andy Stadheim, Ishaan Shandil and Sehoon Kim, Fermentation, GlycoFi/ Merck, Lebanon, NH

Fermentation Process Characterization and Up-scaling of cGMP Hyaluronic acid in Recombinant Bacillus subtilis
Susan Meijer¹, John Larson², Rasmus Devantier², Kristoffer Tømmeraas², Mads Albæk² and Stuart Stocks², (1)Fermentation pilot plant, Novozymes A/S, Bagsværd, Denmark, (2)Novozymes, DK-2880 Bagsvaerd, Denmark

  Optimization of nitrogen sources for protein expression in Escherichia coli
Wout A.M. Wolken and Jolanda Winters-Steenbergen, Global Microbial Applications Lab, Sheffield Bio-Science - a Kerry group business, Almere, Netherlands

Yarrowia lipolytica – A candidate for the production of value-added compounds from alternative carbon sources
Philippe Holt, Jette Thykær, Peter Ruhdal Jensen and Mhairi Workman, Department of Systems Biology, Danish Technical University, Kgs. Lyngby, Denmark

Increased enzyme mediated hydrolysis of sugarcane bagasse through biological pre-treatment with novel lignolytic fungal strains
S.R. Trejo-Estrada, C. Ramirez-López and K.N. Rivera-Hernández, Biotecnología, Centro de Investigación en Biotecnología Aplicada del IPN (CIBA-IPN Unidad Tlaxcala), Tlaxcala, Mexico

Developing media for batch fermentation at microtitre and shake flask scales 
Diane Wilkinson, Lee Blackwell, Ryan Hylands, Malcolm J. Saxton and Rebecca Williamson, Research & Development Department, Novozymes Biopharma UK Ltd, Nottingham, United Kingdom

Screening for optimal fedbatch medium
Sten Erm, Kaarel Adamberg, Raivo Vilu and Reet Tsupilo, CCFFT, Tallinn, Estonia

Feed-In-Time Fed-batch Media for Yeast: Approval with Lipase expressing Pichia pastoris strain in Clone Screening and High Cell Density Fermentation at Microscale
Johannes Hemmerich¹, Frank Kensy¹ and Francisco Valero², (1)m2p-labs GmbH, Baesweiler, Germany, (2)Enginyieria Química, Universitat Autònoma de Barcelona, Barcelona, Spain

Evaluating Streptomyces lividans as potential host for production of heterologous protein
Stig Rattleff, Jette Thykaer and Anna Eilasson Lantz, Department of Systems biology, DTU - Technical University of Denmark, Lyngby, Denmark

Mixing and flow dynamics in a cylindrical shaken bioreactor
A. Ducci and Weheliye Weheliye, Mechanical Engineering Department, University College London, London, United Kingdom

Single-use bioreactors for bacterial fermentation : evaluation of different technologies for cultures of aerobic and anaerobic strains from 3L to 200L scale
Sandy Demay, Grégoire Exbrayat, Nicolas Chaudet, Didier Roze, Catherine Jourdat and Jean-Marc Guillaume, Bioprocess Research and Development Upstream, Sanofi Pasteur, Marcy l'Etoile, France

Nano dosing in bioreactor technology
Philipp Haslebacher¹, Stefan Berger² and Karl-Heinz Selbmann¹, (1)Institute of Print Technology, Bern University of Applied Sciences, Burgdorf, Switzerland, (2)ReseaChem GmbH, Burgdorf, Switzerland

Gradient simulation experiments for targeting population heterogeneity in continuous Saccharomyces cerevisiae fermentation
A.-L. Heins¹, R. Lencastre Fernandes², L. Lundin³, M. Carlquist⁴, T. Johanson¹, S. Sørensen³, V.K. Gernaey² and A. Eliasson Lantz¹, (1)Center for Microbial Biotechnology, Technical University of Denmark, Kongens Lyngby, Denmark, (2)Department of Chemical and Biochemical Engineering, Technical University of Denmark, Denmark, (3)Department of Biology, Copenhagen University, Denmark, (4)Department of Chemistry, Lund University, Sweden

Titer and Recovery Yield Improvement of a Therapeutic Protein Produced in Escherichia coli by Reducing Protein Production Rate
Jianlin Xu, Yueming Qian, Paul M. Skonezny, Li You, Zizhuo Xing, David S. Meyers, Robert J. Stankavage and Zheng Jian Li, Cell Culture and Fermentation Process Development, Bristol-Myers Squibb, Syracuse, NY

Optimization of complete nitrogen sources for a range of microbial applications
Wout A.M. Wolken, E Teunis, J Menton, M van der Woude-Rekers and J Winters-Steenbergen, Global Microbial Applications Lab, Sheffield Bio-Science - a Kerry group business, Almere, Netherlands

Fermentative prodcution of glyceric acid from biodiesel industry byproduct glycerol
Ramanujam Praveen Kumar¹, Balasubramaniyan Bharathiraja², Sivasankaran Chozhavendhan², Subramanian Palani² and Ranganathan Selvaraj², (1)Department of Chemical Engineering, National Institute of Technology,, Mangalore, Karnataka, India, (2)Biotechnology, Arunai Engineering College, Tiruvannamalai, India

Effect of process temperature on fungal morphology in submerged Aspergillus niger processes and its characterization by automated Image Analysis
Panagiotis Sarantinopoulos, Stefaan Breestraat, Rogier Meulenberg and Hein Stam, DSM Biotechnology Center, Delft, Netherlands

Initial Characterization of a Novel Luminescent Oxygen Probe and its Use in Bioreactor Applications
Michael V. Guettler¹, Denise Rumler¹, James Wynn¹, Reza Loloee² and Ruby N. Ghosh³, (1)MBI, Lansing, MI, (2)Physics, MIchigan State University, East Lansing, MI, (3)Physics, Michigan State University, East Lansing, MI

Loss Avoidance:  Realizing efficiency and yield improvement targets in the primary clarification step of high cell density fermentations
D. Gregory Cybulski, Sales, Celeros Separations, Foxboro, MA

Submerged lignocellulose fermentations at high dry matter concentrations
Johan van Groenestijn, Earth, Environmental and Life Sciences, TNO, Zeist, Netherlands and Peter J. Punt, Microbiology & Systems Biology, TNO, Zeist, Netherlands

Temporal analysis of Pichia stipitis proteome using shotgun proteomics as a fermentation monitoring technique
Eric L. Huang and Mark Lefsrud, Bioresource Engineering, McGill University, Ste-Anne-de-Bellevue, QC, Canada

RoboLector Technology – Automated Fermentation Development in Online Monitored Microplates
Oliver Born, Le Wang and Frank Kensy, m2p-labs GmbH, Baesweiler, Germany

Increasing Geldanamycin Production by Medium Modification and Process Improvement
T. Tully, R. Doyle, R. Schild, P. Sharma, M. Gando and A. Goswami, Bristol-Myers Squibb, New Brunswick, NJ

Improving Cell Culture Productivity with a SEG-FLOW™ Automated Online Sampling & Feed System
William Miller¹, Michael Bicksacky¹, Chengbin Lin² and John Knighton³, (1)Flownamics, Inc., Madison, WI, (2)Genzyme, Inc., Framingham, MA, (3)Johnson & Johnson Pharmaceutical Research & Development, Radnor, PA

Optimization of a Saccharomyces cerevisiae fermentation process for production of a therapeutic recombinant protein using a multivariate Bayesian approach
Zhibiao Zu, Microbial and Cell Culture Development (MCCD), , Biopharm R&D, King of Prussia, PA

α-amylase Production by Bacillus Species Isolated From Sweat Food Waste
otman Nasr Ermithi¹, Nagib A. Elmarzugi², Suhaila Naji1³ and Ahmed Shaban Agha¹, (1)Microbiology, Biotechnology Research Center, Tripoli, Libya, (2)Faculty of Pharmacy, Alfateh University, Tripoli, Libya, (3)Microbiology, Biotechnology Reacersh Center, Tripoli, Libya