P19: Increased enzyme mediated hydrolysis of sugarcane bagasse through biological pre-treatment with novel lignolytic fungal strains

Monday, November 7, 2011
Capri Ballroom (Marriott Marco Island)
S.R. Trejo-Estrada, C. Ramirez-López and K.N. Rivera-Hernández, Biotecnología, Centro de Investigación en Biotecnología Aplicada del IPN (CIBA-IPN Unidad Tlaxcala), Tlaxcala, Mexico
Three strains of thermotolerant fungi (CO3-Bag 1, CO1-Bag1 y CO5-B4), isolated from hot compost, were tested for the production of lignolytic enzymes, and selected for their ability to produce lignin peroxidase; manganese peroxidase and laccase.

Sugarcane bagasse, the most abundant agroindustrial lignocellulosic by-product in Mexico, was milled and thermally treated by autoclaving. Conditions of porosity and total solid content of the substrate were optimized for solid fermentation. Steamed bagasse was then treated with a mineral solution and inoculated with each strain, and incubated at 25, 45 or 60 ° C. After either 7 or 15 days of incubation, the material was treated with commercial cellulolytic enzymes Vizcozyme, Celluclast and Pulpzyme.

When compared to untreated controls, the biological pretreatment slightly increased reducing sugar yield (15%), after enzyme treatment of autoclaved sugarcane bagasse, But the referred yield was significantly lower than that obtained when alkaline pretreated bagasse was used.

Further improvements are needed to optimize solid fermentation for the delignification of sugarcane bagasse.

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