Here we describe the evaluation of different single-use bioreactors :
- for anaerobic strain : process was first evaluated at 3L scale in a Cell-ready bioreactor, showing same results as classical glass bioreactor. Then scale-up was successfully implemented at 200L scale in a STR 200 single-use bioreactor.
- For aerobic strain : The Cell-tainer bioreactor has been evaluated with our standard recombinant E.coli process achieving similar biomass and protein production as in stainless steel reactor.
Single-use bioreactors are well adapted for R&D. The main issues (oxygen and heat transfer) linked to aerobic strains such as E.coli were even overcome for the first time in a single-use equipment.