P4: Ethanol production from pretreated eucalyptus wood chips by simultaneous saccharification and fermentation

Monday, November 7, 2011
Capri Ballroom (Marriott Marco Island)
Hibiki Matsushita1, Noriko Yasutani1, Kazunori Habu1, Hirokazu Kikuta1, Hideshi Yanase2, Masakazu Daidai3, Hisayuki Sego4, Yoshiki Yasue4 and Takashi Watanabe3, (1)Biotechnology & Afforestation Business Division, Toyota Motor Corporation, Miyoshi-shi, Aichi, Japan, (2)Department of Chemistry and Biotechnology, Graduate School of Engineering, Tottori University, Tottori, Japan, (3)Research Institute for Sustainable Humanosphere (RISH), Uji, Japan, (4)Japan Chemical Engineering & Machinery Co., Ltd., Osaka, Japan
There is the growing demand to develop highly efficient technology to produce ethanol and chemicals from woody biomass. For enzymatic conversion of lignocellulosic biomass, the pretreatment is necessary. Efficient conversion of the enzymatic hydrolyzates by ethanologenic microbes is also the key factor for ethanol production. To solve them, microwave-assisted solvolysis pretreatment has applied and novel high-performance ethanologenic bacteria are being bred through cell-surface engineering based on genome DNA analysis. In this study, ethanol production from pretreated eucalyptus wood chips using the recombinant cellobiose-utilizing Zymomonas mobilis [pZA-gbgl] was carried out in a 30-L jar fermentor. As a result, over 5% ethanol was produced under the condition of 15% substrate loading.
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