Session 24: Functional Metagenomics
Thursday, July 24, 2014: 8:00 AM-11:30 AM
Regency Ballroom D, Second Floor (St. Louis Hyatt Regency at the Arch)
Convener:
Mark R. Liles - Auburn University, Auburn, LA
This session is focused on heterologous expression of genes recovered directly from microbial assemblages in diverse environments. There are many ways in which the metagenomic DNA may be accessed using next-generation sequencing or cloning of the isolated DNA. Of particular interest are innovative enrichment strategies, cloning systems, heterologous host expression systems, and screening platforms to identify specific functions. This session will include speakers that use a functional metagenomic approach in order to identify previously undiscovered microbial enzymes and metabolites from different environments.


8:00 AM
Functional metagenomics and chromosome engineering for enhanced bioplastics production
Trevor C. Charles, Jiujun Cheng, Ricardo Nordeste, Tam Tran and John Heil, Biology, University of Waterloo, Waterloo, ON, Canada
8:30 AM
Functional soil metagenomics:  elucidation of polycyclic aromatic hydrocarbon degradation potential after 10 years of in situ bioremediation
Márcia Duarte1, Ruy Jauregui1, Thomas Bruls2 and Dietmar H. Pieper1, (1)Microbial Interactions and Processes, Helmholtz Centre for Infection Research, Braunschweig, Germany, (2)Genoscope, Institut de Génomique du CEA, Evry, France
9:00 AM
Identification of polyketide synthase pathways from a large-insert soil metagenomic library
Mark R. Liles1, Jinglie Zhou1, Rosa Ye2, Svetlana Jasinovica2, Megan Niebauer2, Amanda Krerowicz2, Ronald Godiska2, Cheng-Cang Wu2, Blaine A. Pfeifer3 and David A. Mead2, (1)Biological Sciences, Auburn University, Auburn, AL, (2)R&D, Lucigen Corporation, Middleton, WI, (3)Chemical and Biological Engineering, The State University of New York at Buffalo, Buffalo, NY
10:00 AM
Heterologous Cellular Design for Complex Natural Product Support
Blaine A. Pfeifer, Chemical and Biological Engineering, The State University of New York at Buffalo, Buffalo, NY
10:30 AM
Enrichment of novel electroactive bacteria from sediments via potentiostatic growth
Enrico Marsili, Lucinda Doyle and Stefan Wuertz, School of Biotechnology, Dublin City University, Dublin, Ireland
11:00 AM
Culture independent approaches for thediscovery of new bacterial metabolites
Sean F. Brady, Laboratory of Genetically Encoded Small Molecules, Rockefeller University, New York, NY
See more of: Invited Oral Papers