P85 Genome mining of Salinispora sp strains isolated from the São Pedro and São Paulo Archipelago in Brazil
Sunday, January 11, 2015
California Ballroom C and Santa Fe Room
Ms. Karine Pires, Instituto de Ciências do Mar - LABOMAR, pHD student/Universidade Federal do Ceará, Fortaleza, Ms. Michelle Schorn, Center for Marine Biotechnology and Biomedicine, Scripps Institution of Oceanography, pHD student/University of California - San Diego, Dr. Max Crüesemann, Center for Marine Biotechnology and Biomedicine,, Scripps Institution of Oceanography, La Jolla, CA, Mr. Elthon Goes Ferreira, Instituto de Ciências do Mar - LABOMAR, pHD student/Universidade Federal do Ceará, Dr. Letícia Veras Costa-Lotufo, Instituto de Ciências do Mar - LABOMAR and Departamento de Fisiologia e Farmacologia, Professor/Universidade Federal do Ceará and Dr. Bradley S. Moore, Center for Marine Biotechnology and Biomedicine, Scripps Institution of Oceanography, University of California San Diego, San Diego
Studies on marine microorganisms have benefited from the development of DNA sequencing technology combined with bioinformatics-based approaches to the discovery of new secondary metabolites. The genus Salinispora, with a wide geographical distribution, has great potential for the production of secondary metabolites. This genus was recently detected on the coast of Brazil, in the São Pedro and São Paulo archipelago (SPSPA), located on Atlantic Ocean approximately 1,000 km from the continental Brazilian coast (N00º 55.1'  W29º 20.7'). The aim of this study was to explore four new strains (BRA-172, BRA-190, BRA-258 and BRA-201) isolated from SPSPA and characterize their potential for the production of new compounds using genome mining. The 16S RNA sequence identified strain BRA-201 as S. pacifica (e.g. CNS 863), while all other strains were identified as S. arenicola (e.g. CNS 205). Further, genome sequencing of the strains BRA-201, 172 and 258 revealed genome sizes ranging from 6.5 to 7.5MB and analysis using antiSMASH and NaPDoS identified 21-23 gene clusters in each of the strains supporting diverse biosynthetic pathway, including specifically salinosporamide, rifamycin, lymphostin and siderophore related pathways. Although the strains from Brazil do not contain any new gene clusters until now, they have a different subset of clusters as compared with characterized Salinispora strains from elsewhere. Since only a few Salinispora clusters have been chemically characterized to date, we are using molecular network analysis of the extracts to improve the characterization of the strains.