P83 Discovery of secondary metabolites from Bacillus spp. biocontrol strains using genome mining and mass spectroscopy
Sunday, January 11, 2015
California Ballroom C and Santa Fe Room
Christopher Dunlap1, Michael J. Bowman2 and David Schisler1, (1)Crop Bioprotection Research Unit, Peoria, IL, (2)Bioenergy Research Unit, United States Department of Agriculture, Agricultural Research Service, National Center for Agricultural Utilization Research, Peoria, IL
Genome sequencing, data mining and mass spectrometry were used to identify secondary metabolites produced by several Bacillus spp. biocontrol strains. These biocontrol strains have shown promise in managing Fusarium head blight in wheat. Draft genomes were produced and screened in silico using genome mining software. Putative synthesis clusters were confirmed to be functional and the metabolites were structurally characterized with MS/MS techniques.  We identified several known antifungal metabolites as well as antibiotic compounds which have limited efficacy information available. In addition, we documented the presence of several different siderophores, which have been associated with biocontrol activity. This study highlights the advantages of combining these techniques to rapidly characterize new biocontrol strains.