2016 SIMB Annual Meeting and Exhibition

Evaluation of the ambr 15 fermentation system for high cell density XS^TM E.coli and XS^TM P.pastoris: strain screening and scalability
Kevin McHugh¹, J. Betts¹, B. Zoro¹, N. Wagner², J. Rogalla² and C. Kiziak², (1)Sartorius Stedim Biotech Group, Royston, United Kingdom, (2)Lonza AG Visp, Switzerland, United Kingdom

Co-utilization of sugars and lignin-derived compounds by Rhodococcus jostii RHA1 for lipid production
Caixia Wan and Chen Zhu, Bioengineering, University of Missouri, Columbia, MO

A packed bed bioreactor systems for growth of anchorage dependent cells: A comparative study between Eppendorf’s BioBLU^® 5p Single Use Vessel and the Traditional Glass Vessel.
Shannon Guertin, Kamal Rashid and Joseph Duffy, Worcester Polytechnic Institute, Worcester, MA

A first step toward commercial antigen bulk manufacturing in flexible production units
Vincent Colombie, Sanofi Pasteur, Marcy l'Etoile, France

Developing Streptomyces venezualae as a platform organism for production of branched fuel compounds
Eric Sundstrom¹, Setphen Hubbard¹, Satoshi Yuzawa², Leonard Katz², Todd Pray¹ and Deepti Tanjore¹, (1)Advanced Biofuels Process Demonstration Unit, Lawrence Berkeley National Laboratory, Emeryville, CA, (2)QB3 Institute, University of California, Berkeley, Emeryville, CA

Varying nutrient concentrations impact ethanol production during fermentation of cellulosic sugars from mixed feedstock
Chyi-Shin Chen¹, Firehiwot Tachea¹, Akash Narani¹, Matthew Miller¹, Travis Schofield², Phillip Coffman¹, Todd Pray¹ and Deepti Tanjore¹, (1)Advanced Biofuels (and Bioproducts) Process Demonstration Unit (AB-PDU), Lawrence Berkeley National Laboratory, Emeryville, CA, (2)Department of Energy, Science Undergraduate Laboratory Internships (SULI) Program, Emeryville, CA

An all-around commercial oxygen sensor for fermentation off-gas monitoring
Duen G. Mou, Moubio Knowledge Co., Taipei, Taiwan

Differential gene expression in planktonic and sessile cell populations of Clostridium (Ruminiclostridium) thermocellum and their contributions to cellulose bioconversion
Alexandru Dumitrache¹, Dawn M. Klingeman², Mr. Jace Natzke¹, Miguel Rodriguez Jr.¹, Brian H. Davison¹ and Steven D. Brown², (1)Biosciences Division and BioEnergy Science Center, Oak Ridge National Laboratory, Oak Ridge, TN, (2)Biosciences Division and BioEnergy Science Center, Oak Ridge National Laboratory, Oak Ridge National Laboratory, Oak Ridge, TN

Influence of different parameters in the production of natural colorants by Talaromyces amestolkiae in batch fermentation
Valeria de Carvalho Santos-Ebinuma, Department of Bioprocess and Biotechnology, School of Pharmaceutical Sciences, Univ. Estadual Paulista-UNESP, Araraquara, Brazil, Adalberto Pessoa Jr., Department of Biochemical and Pharmaceutical Technology, School of Pharmaceutical Sciences, University of São Paulo, São Paulo, Brazil and Maria Francisca Simas Teixeira, Culture Collection DPUA/UFAM, Universidade Federal do Amazonas, Manaus, Brazil

Production of novel antistreptococcal liamocins by fermentation of agricultural biomass
Timothy D. Leathers¹, Neil P.J. Price¹, Kenneth M. Bischoff¹ and Pennapa Manitchotpisit², (1)Renewable Product Technology, United States Department of Agriculture, Agricultural Research Service, National Center for Agricultural Utilization Research, Peoria, IL, (2)School of Biological Sciences, Illinois State University, Normal, IL

Effects of common defoaming agents on the growth of the endotoxin-free ClearColi^® Escherichia coli host
Kathrine Allikian, Indira Rasiah and Shuguang Zhang, Callaghan Innovation, Lower Hutt, New Zealand

Expression of 2,6-sialyltransferase in Escherichia coli BL21(DE3) and endotoxin-free ClearColi^® under fed-batch fermentation
Kathrine Allikian, Cynthia Sun and Shuguang Zhang, Callaghan Innovation, Lower Hutt, New Zealand

Impact of hydrothermal pretreatments on microalgal biomethane production
Naga Sirisha Parimi and Kesav Das, College of Engineering, University of Georgia, Athens, GA

Transcriptome profiling and phenotypic stability of evolved Saccharomyces cerevisiae strains capable of ethanol production in high solids pine fermentations
Olivia Ann Thompson¹, Gary Matthew Hawkins¹, Steven W Gorsich² and Joy Doran-Peterson¹, (1)Department of Microbiology, University of Georgia, Athens, GA, (2)Biology Department, Central Michigan University, Mount Pleasant, MI

Glycerol fed-batch Pichia pastoris fermentation in the micro-Matrix
Carolina Santos Fernandes¹, Dr. Cristina Bernal Martinez¹, Ann D'Ambruoso², Timo Walvoort¹ and Paul Ter Huurne¹, (1)Applikon Biotechnology, Delft, Netherlands, (2)Applikon Biotechnology, Inc., Foster City, CA

Enzymatic delignification by laccase-natural mediator systems supplemented with xylanase
Caixia Wan and Ying Wu, Bioengineering, University of Missouri, Columbia, MO

Xylitol production by a newly isolated Candida sp. from enzymatic hydrolysate of xylan from black liquor in pulp and paper industry
Sehanat Prasongsuk, Wichanee Bankeeree, Pongtharin Lotrakul and Hunsa Punnapayak, Plant Biomass Utilization Research Unit, Department of Botany, Faculty of Science, Chulalongkorn University, Bangkok, Thailand

Powering fermentations with electroactive microbes
Bhushan Awate and Gemma Reguera, Microbiology and Molecular Genetics, Michigan State University, East Lansing, MI

Production of trehalose from a cellulosic-derived building-block, levulinic acid, by Burkholderia stabilis
H. Habe, T. Fukuoka and T. Morita, Research Institute for Sustainable Chemistry, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Ibaraki, Japan

Impact of aconitic acid on ethanol fermentation
Thomas Klasson, USDA-ARS, New Orleans, LA

Bioethanol production from cassava (Manihot esculenta) peels
Chinwe Christy Isitua¹, Prof. Isaiah Nnanna Ibeh², Elizabeth Umoh³ and Nsikak Daniel¹, (1)Department of Microbiology, College of Sciences, Afe Babalola University Ado-Ekiti (ABUAD), Ado-Ekiti, Nigeria, (2)Department of Medical Laboratory Sciences, College of Medicine, University of Benin, Benin City, Nigeria, (3)Department of Microbiology, Afe Babalola University, Ado-Ekiti, Nigeria

Influence of baffled in the production of natural colorants in orbital shaker
Bruna Regina Zaccarim¹, Maria Francisca Simas Teixeira², Prof. Edwil Aparecida De L Gattas³, Prof. Marcel Otavio Cerri¹ and Valeria de Carvalho Santos-Ebinuma¹, (1)Department of Bioprocess and Biotechnology, School of Pharmaceutical Sciences, Univ. Estadual Paulista-UNESP, Araraquara, Brazil, (2)Culture Collection DPUA/UFAM, Universidade Federal do Amazonas, Manaus, Brazil, (3)School of Pharmaceutical Sciences, Univ. Estadual Paulista-UNESP, Araraquara, Brazil

Pea protein hydrolysate-nutralys pea HF (exp.): probiotic application study
Rongzhu Cheng, Sebastien Maubourguet, Eric Lee, Doug Wenzel, Antoine Billa, Sebastian McCann, Leon Zhou, Thierry Feron and Pascal Leroy, Industrial Application, Roquette America Inc., Geneva, IL

Evaluation of minibioreactors for actinomycetes fermentation for natural product production
Prasanth Maddipati, Jessica Marty, Sheena Becker, Deepti Tyagi, Paul Ketterer, Lori Marcum, Matthew Chase, Mark Mikola, Chris Stowers and Dale Brown, Bioengineering and Bioprocessing R&D, Dow AgroSciences LLC, Indianapolis, IN

Engineering of Rhodococcus jostii RHA1 for consolidated bioprocessing of lignin-derived compounds
Raphael Roccor and Prof. Lindsay D. Eltis, Microbiology and Immunology, The University of British Columbia, Vancouver, BC, Canada

A bifunctional endoglucanase with activity towards carboxymethylcellulose and chitosan from Lysobacter enzymogenes 521
Siraprapa Saraihom¹, Donald Y. Kobayashi², Pongtharin Lotrakul¹, Sehanat Prasongsuk¹, Douglas E. Eveleigh³ and Hunsa Punnapayak¹, (1)Plant Biomass Utilization Research Unit, Department of Botany, Faculty of Science, Chulalongkorn University, Bangkok, Thailand, (2)Department of Plant Biology and Pathology, School of Environmental and Biological Sciences, Rutgers, The State University of New Jersey, New Brunswick, NJ, (3)Department of Biochemistry and Microbiology, Rutgers University, School of Environmental and Biological Sciences, New Brunswick, NJ

Isolation of levoglucosan-utilizing bacteria and crystallographic characterization of levoglucosan dehydrogenase
Ajay Arya, Microbiology, University of Georgia, Athens, GA and Mark A. Eiteman, BioChemical Engineering, University of Georgia, Athens, GA

Characterization of the unique pectinolytic system of Paenibacillus amylolyticus 27C64
Christian Keggi, Microbiology, University of Georgia, Athens, GA and Joy Doran-Peterson, Biomedical and Health Sciences Institute and Microbiology Department, University of Georgia, Athens, GA

Addition of a mixed Bacillus assemblage to commercial banana weaning tanks results in latex remediation, extended tank water viability and a reduction in post-harvest disease
John Gorsuch, Michael Showell and JoElla Barnes, BiOWiSH Technologies, Cincinnati, OH

Enzymatic characterization of six fungal GH5_5 endo-β1,4-glucanases and their performance on corn stover deconstruction
Bingyao Li, Michigan State University, East Lansing, MI and Jonathan D. Walton, Department of Energy Plant Research Lab, Michigan State University, East Lansing, MI

Combinatorial enzyme approach for conversion of agricultural fibers to functional products
Dominic Wong, Mrs. Sarah Batt and Dr. William Orts, Western Regional Research Center, USDA-ARS, Albany, CA

Rational design of E. coli thioesterase I (TesA) for improved specificity towards lauric acid
Nestor Hernandez Lozada¹, Matthew Grisewood², Brian Pfleger¹ and Costas Maranas², (1)Chemical and Biological Engineering, University of Wisconsin - Madison, Madison, WI, (2)Chemical Engineering, Pennsylvania State University, University Park, PA

Studies on simultaneous saccharification and fermentation of cellulosic materials using filtered enzyme and whole culture broth
Yoonsoo Yeo, Nyeshia Daniels, Christiane Ingram and Benedict Okeke, Bioprocessing and Biofuel Reserach Lab (BBRL), Department of Biology, Auburn University at Montgomery, Montgomery, AL

Identification of a soluble phosphatidate phosphatase (PAP) in the developing cotyledons of Gossypium hirsutum
Kandan Sethumadhavan and Heping Cao, Commodity Utilization, Southern Regional Research Center, ARS, USDA, New Orleans, LA

A super lipase: Engineering lipase to increase substrate accessibility by fusion with amphipathic and coiled-coil peptides
Soohwan Lim¹, Kyung Seok Yang², Suk-Chae Jung¹, Dr. Bong Kwan Han¹, Min Sung Kim³, Prof. Sun Chang Kim¹ and Dr. Jun Hyoung Lee³, (1)Intelligent Synthetic Biology Center, Korea Advanced Institute of Science and Technology, Daejeon, South Korea, (2)Biological Sciences, Korea Advanced Institute of Science and Technology, Daejeon, South Korea, (3)KAIST Institute for Biocentury, Korea Advanced Institute of Science and Technology, Daejeon, South Korea

Successful sterile filtration of a squalane emulsion
Angel Lorenzo, SLS Biopharmaceutical Field Operations, Pall Corporation, Pembroke Pines, FL

Improved production of the human recombinant enzime N-acetylgalactosamine-6-sulfatase in Escherichia coli
Luis H. Reyes, Institute for the Study of Inborn Errors of Metabolism, Pontificia Universidad Javeriana, Bogotá, Colombia

The effectiveness of biocides in limiting microbial growth in Louisiana raw sugar mills
Stephanie Boone, Commodity Utilization Research, USDA-ARS-SRRC, New Orleans, LA

Manipulation of plasmid stability through optimization of fermentation conditions
George Chacko, Thomas Li, Mark Berge and Shushil Machhi, Cell Culture & Fermentation Sciences, MedImmune, Gaithersburg, MD

Bioactivities and metabolites of microorganism from special environments of Taiwan
Mei-Huei Chen, Ms. Hsun-Yin Hsu, Dr. Yen-Lin Chen, Ms. Kai-Ping Chen, Ms. Fu-Ling Chan, Mr. Hing-Yuen Chan, Dr. Sung-Yuan Hsieh and Dr. Gwo-Fang Yuan, Bioresource Collection and Research Center, Food Industry Research and Development Institute, Hsinchu, Taiwan

Isotopically nonstationary ¹³C metabolic flux analysis guided strain engineering of isobutyraldehyde producing Synechococcus elongatus PCC 7942
Lara Jazmin¹, Yao Xu², Vi Ern Cheah¹, Adeola Adebiyi¹, Carl Johnson² and Jamey Young³, (1)Department of Chemical & Biomolecular Engineering, Vanderbilt University, Nashville, TN, (2)Department of Biological Sciences, Vanderbilt University, Nashville, TN, (3)Department of Chemical & Biomolecular Engineering/Molecular Physiology & Biophysics, Vanderbilt University, Nashville, TN