Glycerol fed-batch Pichia pastoris fermentation in the micro-Matrix

Pichia pastoris is commonly used for recombinant protein production. Fed-batch fermentation of Pichia strain typically has a growth phase where glycerol is usually used as carbon source, followed by a production phase where methanol serves as inducer and carbon source for protein expression. In the present work fed-batch growth of P. pastoris was investigated at different dissolved oxygen (dO2). The Micro-Matrix system was successfully used to reach high cell density following the protocol previously described by Edwards-Jones et al (2015) in a 7 L Applikon’s bioreactor. We were able to achieve a similar biomass in glycerol fed-batch phase with a dO2 concentration of 100% controlled with air and O2 in cascade. This work shows that micro-Matrix  system can be used in the scale-up process of fed-batch cultures, with reproducible results.