Start | Author Index

P63: Three ways to achieve reproducible pre-cultures in shake flasks

Sunday, August 1, 2010
Pacific Concourse (Hyatt Regency San Francisco)
Tibor Anderlei, Science and Business Development, Adolf Kuhner AG, Birsfelden (Basel), Switzerland and Jochen Büchs, AVT - Chemical Engineering, RWTH Aachen University, Aachen, Germany
3 _ways_pre_cultivation_SIM.pdf
Three ways to achieve reproducible pre-cultures (Escherichia coli) in shake flasks

Reproducible fermentation runs in fermenters are one of the biggest goals in the bioprocess development. One key to achieve this goal is a reproducible pre-culture.

This poster presents three ways to generate reproducible pre-cultures in shake flasks.

  1. Online monitoring of the oxygen / carbon dioxide transfer rate in shake flasks: RAMOS is an online measuring device for shake flask determining the oxygen and carbon dioxide transfer rate.
  2. Online monitoring of the pH and DO: BPM (BioProcess Monitoring) is a device measuring pH and DO in shake flask via optodes.
  3. FeedBeads: FeedBeads are silicon discs containing glucose. FeedBeads enable the user to run a fed-batch in a shake flask very easily. Beside this, FeedBeads can be used to synchronize pre-cultures, resulting in reproducibility.   
See more of: Poster Session 1 Sponsored by Novozymes
See more of: Posters
<< Previous Paper | Next Paper >>