P61: Combination of chitooligossacharides with reduced polimerization degree on cytotoxity of HeLa cells

Chitooligosaccharides are partially hydrolyzed compounds of chitosan that exhibit several biological activities including antitumor, antibacterial and antifungal activity. In this work, we used chitooligosaccharides obtained of Seikagagu Co. (Japan) to investigate the cytotoxicity in HeLa cells. The cytotoxity effect of oligomers of chitosan in HeLa cells was determinated using the MTT method. Chitooligosaccharides used were glucosamine (GlcN), trimers (GlcN₃) and tetramers (GlcN₄), and association between them (GlcN + GlcN₃) and (GlcN + GlcN₄) at different concentrations ranging from 0.12 to 0.96 mg/mL for 72 hours. The compound that showed the higher inhinitory effect on cell proliferation was glucosamine in this case it showed 80% of inhibition.  The trimer did not show any effect on the HeLa cells and tetramer inhibited only 30% of proliferation. The effect of glucosamine combined with trimer and tetramer showed an increase on the inhibition of proliferation of HeLa cells. The mixture of oligomers (GlcN + GlcN₃) and (GlcN + GlcN₄) inhibited cell proliferation on 44 and 50%, respectively.