S16: Biochemical profiling and analysis in process development and scale up to improve overall productivity

Maximizing the production of biofuels, (bio)chemicals and proteins in cell culture/ fermentation systems remains empirical and requires multiple rounds of cause and effect analysis.  Small molecule biochemicals change prior to phenotypic alterations and analysis of these small molecules can provide early indication of the ensuing phenotypic changes, thereby guiding and improving strain design and selection, process development and scale up to improve overall productivity. Metabolon’s  global, untargeted metabolomics platform has been used to gain knowledge of factors affecting cell metabolism at all stages of the bioprocess development and production in a large variety of expression systems including bacteria, yeast, algae and mammalian cells. Case studies will illustrate applications of biochemical profiling in cell line engineering and upstream process development.