Development of an Escherichia coli Recombinant Protein Fermentation Process

This work discusses the development of a high cell density Escherichia coli recombinant protein fermentation process to supply material for toxicological studies. The process development was performed at 10 L scale. Several factors including production host, production phase oxygen uptake rate, glucose feeding, and translational initiation strengths of fragments encoding recombinant protein were evaluated to optimize product titer.  The final process produced an average recombinant product titer of 7.0 ± 0.4 g/L.