In response to the rising demand for high quality, consistent and well-defined hydrolysates, Bio Springer, as the global leader in YE manufacturing, has launched an extensive research project with the primary objective to reduce or eliminate the risks associated with hydrolysates. The focus was to minimize any lot-to-lot variability. Our approach was to perform a comprehensive analytical characterization of a model YE coupled with fermentation studies in order to identify the critical and varying components that have significant impact on the bioprocess performance.
Based on the database we generated both from the monitoring of YE in process parameters and final product analysis we successfully identified a series of YE key components that significantly correlate with growth, yield and several final products quality attributes. We also determined the level of critical components and nutrients required to produce superior growth and productivity in a variety of fermentation models.
This approach represents an important step towards understanding the impact of individual biochemical components on bioprocess robustness and enhancement of production yields. The insights gained from this project will help for the rational design of a new generation of high quality, consistent YE for the fermentation industry.