Cell free protein synthesis platforms capable of manufacturing pipelines of complicated proteins at commercially relevant scales

Sutro’s cell free protein synthesis (CFPS) platforms separate fermentation operations from target protein production and enable innovative approaches to each. Fermentations produce raw materials that are subsequently used to synthesize recombinant protein products in complex cell free biochemical reaction mixes. Sutro has made several advances that have extended the range of products and scale of manufacture for the CFPS technology. Strain engineering has resulted in cell extracts with supplemented levels of key chaperones and isomerases to enhance production of multimeric proteins with many disulfide bonds like full length IgGs. Additional strain engineering has led to extracts capable of incorporating nonnative amino acids into target proteins. These strains can be grown continuously under chemostat conditions to generate large amounts of cell free extract that can be stored until used for CFPS. These advances enable Sutro’s CFPS platforms to manufacture pipelines of complicated products like antibody drug conjugates and bispecific antibodies at commercially relevant scales.