After cloning and expression of the target protein in a host organism and an appropriate screening, a suitable production strain had been identified. During scale-up or by analyzing the results of the first production near fermentation, no product could be detected or only a very small yield. What happened?
Strain developers and process engineers often face the problem that production strains show catabolite repression or overflow metabolism. This dilemma can be circumvented by a process design in fed-batch mode, however the primary screening to gain the appropriate strain still runs in batch mode. The selected clones are not necessarily the best suitable organism for the production process, since screening and production run under different conditions.
PS Biotech is German innovative developer and producer of polymer based release systems to bridge this information gap which allows your company to save time, effort and money. This technique called Feed Plate® bases on standard microplates which easily can be integrated in established cultivation protocols and are designed to be used with standard lab equipment.
Feed Plate® enables high throughput screening to optimize microbial fermentation processes with reliable and adaptive feed rates while no special media or additional enzymes are needed. This allows very easy handling and long storage.
Besides this, the Feed Plate® allows adjusting and synchronization of growth of individual cultures and so leads to reliable screening results.