Collection of Stem cells Using the UniFuge® Single Use Centrifuge

10 liters of myeloid progenitor suspension cells were grown in a Wave bioreactor. The cells were aseptically collected with the UniFuge single use centrifuge.   The viability of the grown cells measured 95%.  The suspension cells were grown for 4 days, doubling each day.  The diameter size of the cells are 15 -20 micron. The cells reached a final cell density 4.93 million cells/ml at time of harvest.   To harvest the cells, the UniFuge centrifuge parameters were feed rate (LPM) and G force .  The cells were layered on a PBS buffer; a manual prefill step was utilized by switching supply lines.    The suspension cells were collected aseptically. After Harvest, the cells were frozen for 24 hours, thawed and viabilities measured