Successful bioprocess scale-up from small scale to large pilot scale using Eppendorf bioprocess systems

The ultimate goal for process development is the realization of large-scale commercial production. Currently, the scale-up of fermentation processes, which is critical to the success of industrial fermentation for bioproduction, are receiving much attention. The fermentation scale-up from benchtop (1 L to 10 L scale) to pilot/production scale (> 100 L) is the important step for effective production of biologicals, including small proteins in the biopharmaceutical market.

Eppendorf bioprocess systems are available in autoclavable, single-use and sterilization-in-place formats. Together, they cover a wide range of working volumes from less than 1 L to as large as 2400 L. The primary scope of this project is to investigate the scale-up capabilities of Eppendorf fermentation systems from small to large scale. Within this presentation, engineering parameters critical for scaling-up fermentation processes are discussed, such as vessel and impeller geometry, tip speed, mixing time, OTR and k_La.