A Novel Glycosylated Macrolide from a Rare Actinomycete, Catenulispora sp. 13F217

Structurally diverse microbial metabolites, especially previously unknown compounds, have significant implications for medical applications and subjects of various researches such as microbial physiology, chemical ecology and chemical biology. Although many compounds have been reported from soil microorganisms and sometimes they tend to be regarded as exhausted resources for novel classes of metabolites, they are still abundant resources in that only a small portion of them have been cultured and chemically investigated. In this respect we isolated more than two hundred actinomycete strains from soil samples collected from Ulleung island, Korea, by various isolation techniques and cultured them on a small scale. On LC/MS screening, one strain named 13F217 arouse our interests because its extract exhibited the relatively large molecular ion peak and the characteristic UV absorbance pattern. The organism was identified as a rare actinomycete, Catenulispora sp. on the basis of 16S rRNA sequence. The pure compound was isolated from culture broth by HPLC and the chemical structure was determined by means of spectroscopic analyses including 1D and 2D NMR experiments and HRESIMS. As a result, it was identified as the novel 26-membered glycosylated macrolide. Further experiments are currently underway to test the biological activities and elucidate the stereochemistry of the new glycosylated macrolide.

Keywords : Secondary metabolites, Catenulispora, macrolide