Induction of 1-hexene monooxygenase activity in immobilized Rhodococcus rhodochrous DAP 96253 cells stabilized in the presence of whole bananas

Alkene monooxygenase (AMO) is widely used in waste disposal industry since it could catalyze the chemical reaction of transferring alkenes into epoxides. Immobilization of enzymes can maintain enzyme activity longer, offer easier enzyme storage conditions and make some enzymes reusable, much research has been done in this area. In this study, Rhodococcus rhodochrous DAP 96253, grown on YEMEA medium supplemented by glucose and urea, was investigated using whole bananas as the inducer of AMO activity and different immobilization methods to maintain this enzyme activity. It was shown that calcium-alginate polyvinyl alcohol (PVA) beads could maintain AMO activity of R. rhodochrous DAP 96253 more stable than calcium-alginate beads. Whole bananas exhibited very obvious effects of inducing AMO activity of R. rhodochrous DAP 96253.