Effects of selected carbohydrates and storage temperatures on 1-hexene monooxygenase activity and stability in cells of Rhodococcus rhodochrous DAP 96253

Rhodococcus rhodochrous DAP 96253, a well-known industrial bacterium, had various alkene monooxygenase (AMO) activities when grown on YEMEA medium supplemented by eight different carbohydrates (glucose, fructose, galactose, sucrose, trehalose, maltose, lactose and maltodextrin) and exhibited different storage temperature and time preferences for maintaining AMO activities. Lactose could induce the most AMO activity of R. rhodochrous DAP 96253 while the cells showed the lowest AMO activity when trehalose was chose for the extra carbon source. AMO activity of R. rhodochrous DAP 96253 at 37°C was not maintained as well as at 4°C and 25°C. Trehalose-induced AMO activity of R. rhodochrous DAP 96253 was more stable from Day 0 to Day 21 at all these three temperatures, compared with the other seven carbohydrates.