Session 8
Enzyme Science and Technology I – Assays and Biochemical Characterization
Tuesday, April 29, 2014: 1:00 PM-4:25 PM
Grand Ballroom F-G, lobby level (Hilton Clearwater Beach)
Cristiane Sanchez - Embrapa, n/a, and Sarah Teter - Novozymes, Davis, CA
Enzyme expression and function requires advanced tools such as sophisticated enzyme assay and characterization techniques. New and improved assay methods and characterization techniques for biomass depolymerizing and debranching enzymes will be included in this session. Progress in understanding and applying oxidative enzymes, expansins, swollenins, and other cell wall disrupting enzymes to improve plant cell wall deconstruction will also be included as well as characterizing and improving enzyme synergy in biomass hydrolysis.

Understanding Free and Complexed Enzyme Mechanisms and Factors Contributing to Cell Wall Recalcitrance
Michael Resch1, Bryon Donohoe1, Peter N. Ciesielski1, Ashutosh Mittal1, Rui Katahira2, Eric Karp2, Michael E. Himmel1, Stephen Decker1 and Gregg T. Beckham2, (1)Biosciences Center, National Renewable Energy Laboratory, Golden, CO, (2)National Bioenergy Center, National Renewable Energy Laboratory, Golden, CO
Enhanced Lignocellulosic Biomass Hydrolysis by Oxidoreductases
Ron Huffman1, Chris Dela Cruz1, Ye Chen1, Terry Green1, Hongzhi Huang2, Feng Xu3, Katja Salomon Johansen4 and Jim Liu1, (1)R&D, Novozymes North America, Franklinton, NC, (2)R&D, Novozymes (China) Investment Co. Ltd., China, (3)R&D, Novozymes, Inc., Davis, CA, (4)R&D, Novozymes A/S, Denmark
Visualizing cellulase activity on the cellulose surface
Bernd Nidetzky and Manuel Eibinger, Institute for Biotechnology and Biochemical Engineering, Graz University of Technology, Graz, Austria
In-situ Rheological Measurements to assess Cellulases for application on Biomass at High Solids Concentrations
Deepti Tanjore1, Phillip Coffman1, Nicole McAffrey2, Julio Baez1 and James Gardner1, (1)Advanced Biofuels PDU (AB-PDU), Lawrence Berkeley National Laboratory, Berkeley, CA, (2)Department of Chemical and Biomolecular Engineering, University of California - Berkeley, Berkeley, CA
Novel structures and functions of lytic polysaccharide monooxygenases
Svein Jarle Horn1, Jane W. Agger1, Trine Isaksen1, Zarah Forsberg1, Roland Ludwig2, Al MacKenzie1, Gustav Vaarje-Kolstad1, Aniko Varnai1, Bjorge Westereng1 and Vincent G. H. Eijsink1, (1)Department of Chemistry, Biotechnology and Food Science, Norwegian University of Life Sciences, Aas, Norway, (2)Department of Food Science and Technology, BOKU, University of Natural Resources and Life Sciences, Vienna, Australia
Quartz crystal microbalance study on enzymatic degradation behavior of hemicellulose in the thin films of lignocellulosic nanofibrils
Akio Kumagai, Biomass Refinery Research Center (BRRC), National Institute of Advanced Industrial Science and Technology (AIST), Higashi-hiroshima, Japan, Takashi Endo, Biomass Refinery Research Center, National Institute of Advanced Industrial Science and Technology, Hiroshima, Japan and Seung-Hwan Lee, Department of Forest Biomaterials Engineering, Colleage of Forest and Environmental Sciences Kangwon National University, Chunchen, South Korea
Influence of temperature on kinetic parameters for hydrolysis of insoluble cellulose by Cellobiohydrolase Cel7A
Trine H. Sørensen1, Nicolaj Bagger1, Michael S. Windahl1, Nina Lei1, Johan P. Olsen1, Jeppe Kari1, Kim Borch2 and Peter Westh1, (1)Nsm, Roskilde University, Roskilde, Denmark, (2)Protein Biophysics, Novozymes A/S, Bagsvaerd, Denmark
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