The novel mesoporous silica aerogel modified with protic ionic liquid for lipase immobilization

The mesoporous silica supports (aerogels) was used to immobilized Burkholderia cepacia lipase (BCL) by physical adsorption (PA or PAIL), covalent binding (CB or CBIL) and encapsulation (ENC or ENCIL) method in the absende or presence of protic ionic liquid N-methylmonoethanolamine pentanoate (PIL). Yield immobilization (Ya) and operational stability was determined by hydrolysis reaction of olive oil. The biocatalysts were characterized morphologicaly (nitrogen adsorption - BET method and scanning electron micrographs – SEM) and physical-chemically (Fourier Transform Infrared Spectroscopy - FTIR and thermogravimetric analysis – TG). The Ya (37% to 83% by adsorption physical) and operational stability (2 to 23 batches by encapsulation) increased when utilized support modified with PIL. For derived immobilized observed by BET method that LIP have any influence of the pores of silica, in this case ADS and CB for ADSIL and CBIL was observed increase pores size possible due the higher amount of BCL immobilized that determined in the Ya and operational stability probably caused by the entrance of the enzyme on the pore of structure silica aerogel. SEM image shows changing the structure and proprieties of immobilized derived with PIL. Characteristic band FTIR was obtained silanol groups and amides I, IV and V, showing too efficiency of the immobilization of BCL in methods studied. Thermogravimetric curves showed higher variation of loss of the mass after the modification of silica in the presence PIL for immobilization derived. Therefore, biocatalysts most efficient were ADSIL considering yield immobilization and ENCIL for operational stability.