Use lipase from Burkholderia cepacia immobilized onto mesoporous silica modified with protic ionic liquid for production ethyl ester

A novel mesoporous silica material was obtained sol-gel technique and modified using ionic liquid as additive hydrophobic and strategies for improve efficiency of biocatalyst immobilized. In this study was used immobilized lipase from Burkholderia cepacia onto mesoporous silica by physical adsorption method in presence protic ionic liquid, N-methylmonoethanolamine pentanoate (IL), for production ethyl ester. Immobilized biocatalyst (20% w/w) was used in the transesterification reaction of colza oil and ethanol (molar ratio 1:7) at 40ºC, 80 rpm and different times (8 up to 120h). Mesoporous silica and immobilized enzymes were characterized using nitrogen adsorption (BET method), Fourier transform infrared (FT-IR), thermal analysis (TGA) and scanning electron microscopy (SEM). Observed that the biocatalyst immobilized onto mesoporous silica by physical adsorption method in presence protic ionic liquid converted 82% of ethyl esters at 120h and the biocatalyst immobilized onto mesoporous silica in absence protic ionic liquid only converted 68%. It was shown that the introduction of IL influenced the catalytic behavior of lipase from Burkholderia cepacia significantly by changing the structure and surface properties of silica. Therefore, this results shows that immobilized lipase onto matrix produced by the sol-gel technique modified with the additive ionic liquid protic can play an important role in their biodiesel production.