8-27: Co-production of cellulose nanocrystal and monosaccharides from disk milled biomass by enzymatic hydrolysis

Tuesday, May 1, 2012
Napoleon Ballroom C-D, 3rd fl (Sheraton New Orleans)
Ricardo Sposina Sobral Teixeira1, Ayla Sant'Ana da Silva1, Jae-Hyuk Jang2, Shanqing Liang3, Han-Woo Kim3, Kazuhiko Ishikawa3, Takashi Endo3, Elba P.S. Bon1 and Seung-Hwan Lee3, (1)Biochemistry Department, Chemistry Institute, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil, (2)Department of Forest Biomaterials Engineering, College of Forest and Environmental Sciences, Kangwon National University, (3)Biomass Technology Research Center, National Institute of Advanced Industrial Science and Technology, Higashi-Hiroshima, Japan
This work addressed the coproduction of cellulose nanocrystal (CNs) and monosaccharides via the separate enzymatic hydrolysis of pure cellulose, Eucalyptus holocellulose, wood pulp and sugarcane bagasse which were previously fibrillated by disk milling. Nevertheless acidic hydrolysis has been reported for the production of CNs the use of endoglucanases, which have the ability to hydrolyze the amorphous cellulose regions, allows the advantageous adjustment of the aspect ratio of the cellulose nanocrystals. Enzymatic hydrolysis experiments (10 mg/mL substrate concentration) were conducted using the commercial enzyme OptimashTMBG (Genencor International, USA) and thermophilic enzymes (endoglucanase from Pyrococcus horikoshii and β-glucosidase from Pyrococcus furiosus). The physical properties and morphology of fibrillated biomass and the residual saccharification product were analyzed by X-ray diffraction, the measurement of the specific surface area (SSA), Atomic Force Microscopy and Scanning Electron Microscopy. Disk milling treatment resulted on the formation of long and twisted nanofibers with 15–30 nm wide which changed into shorter and straighter microfibers, mixed with CNs, in the residue of the enzymatic hydrolysis. The aspect ratio of the cellulose fibers, which depended on the degree of the enzymatic hydrolysis, was successfully adjusted. Enzymatic saccharification resulted on the increase of SSA and crystallinity. Glucose concentrations varied within 1.0 - 7.4 mg/mL, depending on the enzyme and biomass type. The use of Optimash on holocellulose, sugarcane bagasse and pulp also allowed the detection of 0.36 to 1.65 mg/mL of xylose.
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