9-07: Bioconversion of biodiesel derived crude glycerol using Clostridium pasteurianum immobilized on reticulated polyurethane foam

Tuesday, May 1, 2012
Napoleon Ballroom C-D, 3rd fl (Sheraton New Orleans)
Swati Khanna, Arun Goyal and Vijayanand Suryakant Moholkar, Center for Energy, Indian Institute of Technology, Guwahati, Guwahati, India

The conversion of biodiesel derived crude glycerol to n-butanol via anaerobic fermentation pathway, using polyurethane foam immobilized Clostridium pasteurianum cells was studied. Clostridium pasteurianum cells convert glycerol to n-butanol, 1,3-propanediol and ethanol. 10 g/L biodiesel derived crude glycerol as a substrate in buffered media produced 4.32 g/L 1,3-propanediol, 3.85 g/L butanol and 1.61 g/L ethanol. The cells were reused for 5 consecutive cycles and showed significant reusability. The activity for butanol production decreased to half, one third for 1,3-propanediol while the activity for ethanol production became zero after 5 cycles. This is the first report of immobilization of C. pasteurianum cells on reticulated polyurethane foam and their employment in 1,3-Propanediol, butanol and ethanol production from crude glycerol containing feedstock. The results indicate that the biodiesel derived crude glycerol is a valuable feedstock for production of commodity chemicals as well as future fuels.

Keywords: Biobutanol, 1,3-Propanediol, Clostridium pasteurianum, Immobilization

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