Monday, May 2, 2011
Grand Ballroom C-D, 2nd fl (Sheraton Seattle)
Miscanthus is referred one of an ideal cellulosic bioenergy crop which can be used to generate biofuel, heat and power, and relieve carbon dioxide emissions. Miscanthus plant was named as 'Geodae-Uksae #1' which is newly collected from damp lands of southern Korea. It shows greater biomass productivity than those of normal Miscanthus species (M. sinensis and M. sacchariflorus). This study firstly reports the development of a specific, sensitive, and reproducible sequence characterized amplified region (SCAR) marker to distinguish 'Geodae-Uksae' species from the other native Miscanthus species. A putative 1.8 kb polymorphic sequence, specific to 'Geodae-Uksae #1' was identified by randomly amplified polymorphic DNA (RAPD) N8018 primer. Furthermore, SCAR markers (Geodae 1-F and Geodae 1-R) were designed from the unique RAPD amplicon. The SCAR primers produced a specific 1,799-bp amplicon in authentic 'Geodae-Uksae #1', and not in the allied species. This specific SCAR marker could be contributed to the identification method of 'Geodae-Uksae #1', which can be the main cellulosic biomass source of 2nd generation’s bioethanol in the future.