Tuesday, April 20, 2010
10-28

Comparison of the effect of enzymatic extracts from Thermoascus aurantiacus and Trichoderma reesei on sugarcane bagasse saccharification

Thiago Okubo Procópio Pinto1, Maurício Boscolo2, Eleni Gomes3, and Roberto Da Silva2. (1) Food Engineering, UNESP, Rua Cristovao Colombo, 2265, São José do Rio Preto, Brazil, (2) Chemistry and Environmental Science, UNESP, Rua Cristovao Colombo, 2265, São José do Rio Preto, Brazil, (3) Biology, UNESP, Rua Cristovao Colombo, 2265, São José do Rio Preto, Brazil

Cellulose is the most abundant organic compound on earth and a renewable energetic source for producing alternative fuels such as ethanol. The biological conversion of biomass usually involves several sequential steps: lignocellulose pretreatment, enzymatic cellulose hydrolysis, and fermentation. In this work, the fungi Thermoascus aurantiacus CBMAI756 and Trichoderma reesei QM9414 were cultivated by solid-state fermentation (SSF), in sugar cane bagasse and wheat bran (1:1), at 50 °C for 120 hours and 28 °C for 216 hours, respectively. The enzymatic extracts were evaluated for CMCase, Xylanase and β-glucosidase activities. The following results were obtained for T. aurantiacus and T. reesei, respectively (U/g): 535 and 54; 3881 and 640; 14 and 3.5. The enzymatic hydrolysis of sugar cane bagasse was carried out at 60 and 40 °C, and 2% consistence.  The release of reducing sugars increased with the increase of the time reaction reaching a maximum of 7.2 and 9.3%, for the thermophilic and the mesophilic microorganism, respectively. The data suggest that an enzymatic cocktail with both extract might be a promising strategy for future works on the sugar cane hydrolysis.

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