Tuesday, April 20, 2010
10-21
Application of cellulase from solid state fermentation in sugar cane bagasse pretreated by ionizing radiation or steam explosion
The effectiveness of sugar cane bagasse pretreated by steam explosion or ionizing radiation was evaluated by means of enzymatic hydrolysis yield, on the basis of glucose release. The irradiation was performed using the Radiation Dynamics Electron Beam Accelerator with 1.5 MeV and 37 kW, applying absorbed doses from 30 to 150 kGy.
Cellulases from solid state fermentation of a novel fungus, Myceliophthora M77, were applied to sugar cane bagasse at 10 FPU/gdm, pretreated by: (1) steam explosion; (2) ionizing radiation at 30 kGy; (3) ionizing radiation at 70 kGy; (4) ionizing radiation at 150 kGy.
Hydrolysis runs were performed as follows: to 1g of dry pretreated substrate, 10 FPU were added (extract from SSF) in acetate buffer at pH 5.0 in order to reach 1% of solids in the medium. Besides, sodium azide (32mg/L) and cyclohexane (10mg/L) were added to prevent bacterial and fungal growth. Flasks with this medium were put in a shaker at 180 r.p.m., 50C, and, samples were withdrawn periodically.
*: glucose produced relative to the total amount of glucose in cellulose.
Sugar cane bagasse pretreated by ionizing radiation showed better results than the bagasse pretreated by steam explosion. Hydrolysis of sugar cane bagasse pretreated by ionizing radiation at 30 kGy reached the highest yield within 6 hours of reaction at 50C, relative to the higher doses applied.
Cellulases from solid state fermentation of a novel fungus, Myceliophthora M77, were applied to sugar cane bagasse at 10 FPU/gdm, pretreated by: (1) steam explosion; (2) ionizing radiation at 30 kGy; (3) ionizing radiation at 70 kGy; (4) ionizing radiation at 150 kGy.
Hydrolysis runs were performed as follows: to 1g of dry pretreated substrate, 10 FPU were added (extract from SSF) in acetate buffer at pH 5.0 in order to reach 1% of solids in the medium. Besides, sodium azide (32mg/L) and cyclohexane (10mg/L) were added to prevent bacterial and fungal growth. Flasks with this medium were put in a shaker at 180 r.p.m., 50C, and, samples were withdrawn periodically.
| Sugar Cane Bagasse Pretreated as described | (1) | (2) | (3) | (4) |
| Hydrolysis Yield* (%) in 6h | 7.8 | 13.5 | 10.6 | 9.6 |
Sugar cane bagasse pretreated by ionizing radiation showed better results than the bagasse pretreated by steam explosion. Hydrolysis of sugar cane bagasse pretreated by ionizing radiation at 30 kGy reached the highest yield within 6 hours of reaction at 50C, relative to the higher doses applied.
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See more of The 32nd Symposium on Biotechnology for Fuels and Chemicals (April 19-22, 2010)
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See more of The 32nd Symposium on Biotechnology for Fuels and Chemicals (April 19-22, 2010)