Tuesday, April 20, 2010
11-50

Lignocellulose fermentation by the alternative industrial ethanol yeast Dekkera bruxellensis

Johanna Blomqvist, Johan Schnürer, and Volkmar Passoth. Dept. of Microbiology, Swedish University of Agricultural Sciences (SLU), Box 7025, SE-750 07 Uppsala, Uppsala, Sweden

Dekkera bruxellensis is an alternative industrial ethanol production yeast. In its industrial environment it can cope with high numbers of lactic acid bacteria. Its ethanol productivity was not changed in a pH- and temperature interval from 3-5 and 25-37°C. Due to their lower glycerol production, industrial D. bruxellensis isolates were more efficient in terms of ethanol and biomass yield compared to two industrial Saccharomyces cerevisiae strains. Since this yeast grows slower than S. cerevisiae it is especially appropriate for systems running continuously with cell recirculation.
Ethanol production from lignocellulosic biomass using D. bruxellensis is poorly investigated. Our isolates were able to ferment cellobiose to ethanol. Lignocellulose has a poor content of nitrogen sources and the ability of some D. bruxellensis strains to assimilate nitrate may be advantageous for ethanol production.
We tested the inhibitor tolerance of D. bruxellensis and the influence of adding ammonium on its ability to produce ethanol from an aspen sawdust hydrolysate. Finally, ethanol productivity in a continuous fermentation with cell recirculation was tested. The results were compared with those obtained with an industrial S. cerevisiae isolate.