Tuesday, April 20, 2010
11-23

Efficiency of sugar-to-ethanol conversion by different Saccharomyces cerevisiae strains during raspberry must fermentation

Whasley F. Duarte1, Giuliano M. Dragone2, Disney R. Dias1, José M. Oliveira2, José A. Teixeira2, and Rosane F. Schwan1. (1) Biology Department, Federal University of Lavras, Campus Universitário, Lavras, Brazil, (2) IBB - Institute for Biotechnology and Bioengineering, Centre of Biological Engineering, University of Minho, Campus de Gualtar, 4710-057, Braga, Portugal

The use of selected yeasts for winemaking gives multiple advantages over the traditional spontaneous fermentation. There is general agreement among experts that these yeasts should show low production of volatile acidity, high tolerance to alcohol, ethanol production according to the amount of sugar present in the must, total fermentation of sugars, high fermentation rate, growth at high temperature, low hydrogen sulfide production, low foaming, killer phenotype, low acetaldehyde production and good glycerol production. The objective of this study was to evaluate the production of ethanol by different Saccharomyces cerevisiae strains from raspberry must. Yeasts were grown in raspberry must with sugar content adjusted to 16º Brix with sucrose syrup and pH 4.0 adjusted with calcium carbonate. Batch fermentations (100 ml) were carried out at 22ºC. Sucrose, glucose, fructose and ethanol were determined by HPLC. The kinetics parameters, ethanol yield factor (Yp/s), fermentation efficiency (Ef), % of substrate conversion to ethanol and ethanol productivity (Qp) were calculated. S. cerevisiae VR-1 gave the highest Yp/s (0.49 g/g), followed by the strains UFLA FW 15 and CAT-1 (0.48 g/g). Among the yeast strains tested, S. cerevisiae UFLA CA 155 had the lowest Ef (77.5%) and S. cerevisiae VR-1 achieved the highest value of Ef (96%) at the end of fermentation. All the yeasts, except S. cerevisiae UFLA FW 1162, showed high values (> 97%) of conversion of substrate to ethanol. S. cerevisiae UFLA FW 15, VR-1 and BG showed the highest Qp of 1.49 g.l-1.h-1. The lowest Qp (1.15 g.l-1.h-1) was found for the strain UFLA FW 1162. It can be concluded that S. cerevisiae VR-1 showed the best performance for ethanol production during raspberry must fermentation.

See more of Poster Session Two
See more of General Submissions

See more of The 32nd Symposium on Biotechnology for Fuels and Chemicals (April 19-22, 2010)