Tuesday, April 20, 2010
10-46

Cellulase and amilase production by Aspergillus niger in solid state processes using fixed-bed column reactors and waste from rice and passion fruit production

Christiane P. Rocha1, Ana B. C. Rodrigues1, Carlos A. V. Almeida1, Vicelma Luiz Cardoso2, and U. Coutinho Filho2. (1) Faculdade de Engenharia Química, Universidade Federal de Uberlândia, Av. João Naves de Ávila 2121-bloco 1K213, Uberlândia, Brazil, (2) Faculty of Chemical Engineering, Uberlândia Federal University, P.O. Box 593, CEP 38400-902, Uberlândia, Brazil

Now days the concepts of minimization, recovery, by-products utilization and bioconversion of waste are necessary to achieve long-term sustainable agrobusiness practices. Despite this situation, a large fraction of biomass from section agrobusiness and activities primarily associated with food production generate waste products or by-products that require costly disposal. These unused biomass represents lost of fuel, lost of high value nutrients and generate pollution. The purpose of this paper was to evaluate biomass exploitation to produce amylase and celullase under solid state fermentation (SSF) by Aspergillus niger. The fermentations were proceeded under previously determinated conditions represented by the concentration of 50% (vol%) of rice waste biomass ,50% ( vol% ) of passion fruit biomass , 0.25% of cell (wt% ) and 50% of water ( wt%). The response surface methodology (RSM) was used to optimize the amylase and celullase production in 50 cm fixed bed bioreactor. According to the results obtained, the cellulase production increases with increasing temperatures, increases with air flow increasing, decreses with bed height increasing and the best cellulase results were: bed height in the range of 10-15 cm, air flow in the range between 0.80 and 2.0 vvm, temperature in the range of 42- 45 oC. For amylase production, the best bed height was in the range between 40-45 cm, the air flow was in the range between 0.60 and 1.3 vvm, the enviroment temperature in the range of 32- 37 oC.

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