Tuesday, April 20, 2010
10-37

Saccharolytic enzymes production from disk milled rice straw by Acremonium cellulolyticus

Akihiro Hideno, Hiroyuki Inoue, Kenichiro Tsukahara, Shinichi Yano, Xu Fang, Takashi Endo, and Shigeki Sawayama. Biomass Technology Research Center, National Institute of Advanced Industrial Science and Technology, 2-2-2 Hiro-suehiro, Higashi-hiroshima, Japan

Recently, rice straw which can be a potential source for ethanol production has been attracted a lot of interest in Asian countries. On-site saccharolytic enzymes production that utilizes the part of target lignocellulosic material for bioethanol production as a cheap carbon source has been suggested to reduce the cost of enzymes and the storage time. In this study, the saccharolytic enzymes production using the rice straw pretreated with various methods as carbon source was investigated using Acremonium cellulolyticus strain, which is a hyper cellulase enzyme producing strain. The rice straw cut out to less than 3 mm by the cutter mill was treated by wet disk milling, ball milling and hot compressed water treatment. These pretreated rice straw and commercial cellulose (Solka floc) were used as carbon sources for cellulase production by the fungus A. cellulolyticus. FPase activities obtained from ball milled and disk milled samples were as much high as that in Solka floc. On the other hand, the xylanase and xylosidase activities obtained from the ball milled and the disk milled samples were higher than those in Solka floc. When the hot-compressed water treated rice straw was used, there were no enzyme activities because of inhibitors generation. The culture from the disk milled rice straw was more suitable for the enzymatic hydrolysis of pretreated rice straw than that from Solka floc. The disk milling could be one of the excellent pretreatment not only to provide substrate for enzymatic hydrolysis but also to supply the economical carbon source for the cellulase production. This work was supported by the Resional Biomass Energy Project, Ministry of Agriculture, Forestry and Fisheries, Japan.

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