Tuesday, April 20, 2010
8-24
Cellulolytic enzymes production by submerged culture using pre-treated lignocellulosic agro-industrial residues as substrate
Trichoderma reesei ATCC 2768 was grown in submerged culture for cellulase production using as substrates the following lignocelulosic residues: cashew peduncle bagasse and coconut bagasse. These substrates were tested by three different routes: (1) not treated, (2) treated with NaOH (1M) and (3) with high pressure explosion. The results showed that T.reesei ATCC 2768 presented greater cellulase production in a culture containing cashew peduncle bagasse treated with NaOH (2.16 IU / mL of CMCase and 0.21 IU / mL FPase), where the convertion of the cellulose in total reducing sugar (TRS) form was 98%, compared to the high pressure explosion treatment (0.85 IU / mL of CMCase and 0.17 IU / mL FPase), which presented 62% of TRS. The cashew peduncle bagasse without treatment showed a lower activity compared to the treated bagasse (0.53 IU / mL of CMCase and 0.15 IU / mL FPase). Cellulase production is lower in culture containing coconut residue treated with 1M NaOH (0.48 IU / mL of CMCase and 0.07 IU / mL FPase) and reached 49.9% convertion. Coconut bagasse without treatment did not present enzymatic activity. The cell concentrations to both substrates were greater when using NaOH (1 M ) treatment than high pressure explosion one. It was observed that the cashew peduncle bagasse is a potential inducer to the cellulolytic enzymes synthesis, presenting better performing than coconut bagasse. It was also observed that the treatment improves enzymes synthesis process.Key-words: cashew peduncle bagasse; coconut bagasse; Trichoderma reesei ATCC 2768; cellulases
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See more of The 32nd Symposium on Biotechnology for Fuels and Chemicals (April 19-22, 2010)
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See more of The 32nd Symposium on Biotechnology for Fuels and Chemicals (April 19-22, 2010)