Tuesday, April 20, 2010
11-60

Effects of lignocellulosic inhibitory compounds and hydrolysates on ethanol fermentation by Saccharomyces cerevisiae K35

Hawon Lee1, Pil-Jae Seong1, Dae Haeng Cho1, Yong Hwan Kim1, Sung Bong Kim2, Jong Ho Lee2, Seung Wook Kim2, and Chulhwan Park1. (1) Department of Chemical Engineering, Kwangwoon University, 447-1 Wolgye-Dong, Nowon-Gu, Seoul, 139-701, South Korea, (2) Department of Chemical and Biological Engineering, Korea University, 1 Anam-dong, Sungbuk-ku, Seoul, 136-701, South Korea

In the ethanol fermentation using lignocellulose, hydrolysis process of lignocellulose to make the fermentable sugars is required. However, inhibitory compounds decreasing the fermentability of microorganism are formed during hydrolysis. The performance of Saccharomyces cerevisiae K35 in the medium including inhibitors such as acetic acid, furfural, 5-hydroxymethylfurfural (5-HMF), syringaldehyde, and coumaric acid as well as in the yellow poplar and wasted wood hydrolysates was investigated. The cell growth was inhibited with the increase of acetic acid concentration but acetic acid showed no inhibition on ethanol production. Furfural and 5-HMF considerably decreased the cell growth and ethanol production in the over 3 g/L concentration. Syringaldehyde and coumaric acid displayed a similar pattern of those of furfural and 5-HMF. In the 5 g/L syringaldehyde and coumaric acid S. cerevisiae K35 produced 1-2% of ethanol production of the reference culture. In the fermentation of yellow poplar and wasted wood hydrolysates, there was no significant synergetic effect of multiple inhibitory compounds.

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