All strains performed better in conditioned hydrolysate than in hydrolysate that was only neutralized. Although for yeast fermentations, the performance differences were minor. The Saccharomyces cerevisiae yeast strains D5A, a Broin commercial yeast, and a yeast obtained from ATCC (#26602) performed well in a 85% neutralized hydrolysate converting glucose to ethanol at yields of 67%, 68%, and 71%, respectively. A recombinant Zymomonas mobilis 8b achieved ethanol yields in neutralized and conditioned hydrolysate of 68% and 74% in 40% hydrolysate, respectively, but only 17% and 41% in 85% hydrolysate, respectively. High ethanol concentrations (85 – 90 g/L) were produced by the Broin yeast and ATCC #26606 in glucose supplemented hydrolysate achieving ethanol yields of 70.4 % and 73.8%, respectively.