Sunday, April 29, 2007
1A-23

Near Infrared Spectroscopy (NIRS) as a feedstock selection tool

Thomas H. Ulrich1, Corey W. Radtke2, Peter A. Pryfogle2, Daniel M. Stevens3, and J. Richard Hess4. (1) Biofuels & Renewable Energy Technologies, Idaho National Laboratory - Battelle Energy Alliance, 2525 North Freemont Avenue, IRC - 602/MS2203, Idaho Falls, ID 83415, (2) Biological Sciences, Idaho National Laboratory - Battelle Energy Alliance, 2525 North Freemont Avenue, IRC - 602/Mail Stop 2203, Idaho Falls, ID 83415, (3) BYU - Idaho Student, Idaho National Laboratory - Battelle Energy Alliance, 2525 North Freemont Avenue, IRC - 602/Mail Stop 2210, Idaho Falls, ID 83415, (4) Renewable Energy & Power, Idaho National Laboratory - Battelle Energy Alliance, 2525 North Freemont Avenue, IRC - 602/Mail Stop 2210, Idaho Falls, ID 83415

Plant breeding programs can benefit from selection tools that can identify genotypes with important agronomic and bioenergy traits.  The lack of rapid selection tools for composition and ethanol potential makes breeding for feedstock quality by conventional tools costly when screening large populations of cultivars.  Near infrared spectroscopy (NIRS) has demonstrated the ability to reduce labor, time and cost associated with laboratory chemical analysis of biomass.  NIRS is an indirect measurement of plant composition so it must be calibrated with suitable representatives of the genomic population.  Even though this calibration is initially a major effort, once calibrations are made and correlated with feedstock composition, subsequent screening of large populations is rapid and less costly.   Therefore, NIR spectra were collected from mature plants that were dried and ground to pass through a 2mm screen.  The material was analyzed using a FOSS 6500 NIR spectrophotometer.  Collected spectra were compared by principal component analysis (PCA), a statistical method in WinISI.  Only those materials that represented the greatest portion of the population were selected for further chemical analysis.     

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