Monday, April 30, 2007

Oligosaccharide synthesis from cellobiose

Misook Kim, Department of Food Science, Louisiana State University, Baton Rouge, LA 70803 and Donal F. Day, Audubon Sugar Institute, LSU Agricultural Center, 3845, Hwy 75 St, St. Gabriel, LA 70776.

Cellobiose is a potential product from a biorefinery. Conversion of cellobiose to a higher value product will positively impact the economics of biomass conversion. There is a growing market for oligosaccharides in food and pharmaceutical industries for sweeteners, prebiotic compounds, anti-cariogenic and immuno-stimulating agents. A transglycosylation reaction of cellobiose as an acceptor with sucrose as a donor was catalyzed by a dextransucrase produced from Leuconostoc mesenteroides B-512FMCM. A novel trisaccharide, α-D-glucopyranosyl-(1→6)-β-D-glucopyranosyl-(1→4)-D-glucopyranose was produced. This trisaccharide appears to have a number of valuable functional properties. A lab scale process for production of this material has been developed. Several factors, such as the concentration and the ratio of sucrose to cellobiose, the concentration of enzyme, reaction times, pH, and temperature have been tested. Scale-up and production of large quantities of the material will all testing in a range of applications and may lead to a new market for a lignocellulose based product.