In view of this we isolated one microorganism from the soil and that was identified to be closely related to Bacillus subtilis subsp. subtilis ATCC 6051 on the basis of partial 16S rRNA gene sequence homology. We applied statistical method, Plackett-Burman design to screen and select the best carbon and nitrogen sources for the production of alkaline protease. From these experiments we selected four components as significant factors, starch, corn steep liquor, yeast extract and inoculum size. From the preliminary experiments 550 U/ml protease could be obtained. In the next stage we are planning to enhance the protease production by optimizing the culture conditions and medium composition through a central composite design of response surface methodology.