Sunday, April 29, 2007
1B-61

Screening of Xanthomonas sp for production of xanthan gum

Ieda Rottava1, Grasiela Batesini2, Rogério L. Cansian2, Octavio Augusto Ceva Antunes3, and Francine F. Padilha4. (1) Bioquímica, Universidade Federal do Rio de Janeiro, Instituto de Química, CT, Bloco A, Lab. 641, Rio de Janeiro, 21.945-970, Brazil, (2) Ciências Agrárias, URI - Campus de Erechim, Av. Sete de Setembro, 1621, Erechim, 99700-000, Brazil, (3) Departamento de Bioquímica, Universidade Federal do Rio de Janeiro, Instituto de Química, CT, Bloco A, Lab. 641, Rio de Janeiro, 21945-970, Brazil, (4) Saude e Meio Ambiente, UNIT, Av Murilo Dantas, 300, Aracajú, 49032-490, Brazil

Xanthan gum, synthesized for Xanthomonas sp, is a polysaccharide of raised commercial interest, mainly for the industry of foods, druggist and fuels, for its raised viscosity in low concentrations and ample band of stability the temperature and pH. The objective of the work was the screening of Xanthomonas sp isolated in Brazil for production of xanthan gum, evaluating the productivity and apparent viscosity of the watery solutions. The production of biopolymer was carried through in the  MPI + II media, 28ºC, 180 rpm, 96 hours. The fermentation broth was centrifuged the 5500 rpm for 40 minutes and the separate gum for addition of etanol 95% in the ratio of 1:3 (v/v). The two strains with better productivity (8,83 and 7,97 g.L-1) and apparent viscosity (1818 and 925 mPas.s-1) had been respectively Xanthomonas campestris pv. mangiferaeindicae and Xanthomonas axonopodis pv. Manihotis.