Sunday, April 29, 2007
1B-60
Screening of microorganisms for limonene bioconversion
From the allyl
oxidation of the limonene, it is possible to get oxygenates composites as carveol and carvone. Different
isomers of these have property of flavor of mint, cumin or anise, of interest
for the industries of cosmetics, druggist and foods. The objective of this work
was to carry through screening of microorganisms, from rejects of the citric
juice industry, and other sources of limonene, for bioconversion of this. The
election was carried through with the isolation of diverse microorganisms in
plates with Potato Dextrose Agar. To verify the enzyme presence that degrades
limonene in the isolated microorganisms, it was used minimum media more
limonene, in electromagnetic agitation. Fermentation in orbital agitator was
become fulfilled, using broth Potato Dextrose and limonene (1.5%, v/v), with
the microorganisms that had presented resulted positive in the previous test.
The products gotten in the bioconversion process (dehydrocarveol
and cis-carveol, among others), had been
characterized by GC/MS.
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