Monday, April 30, 2007
6-49

High cell density culture of Schizochytrium to produce DHA with a “Shift” strategy

Zhanyou Chi1, Bo Hu2, Yan Liu3, and Shulin Chen3. (1) Biological System Engineering, Washington State University, L.J. Smith 213, Pullman, WA 99163, (2) Department of Chemical Engineering, University of Puerto Rico Mayaguez, Rd 108 Km 1.0, Mayaguez, PR 00681-9046, (3) Department of Biological System Engineering, Washington State University, L.J. Smith 213, Pullman, WA 99163

Producing high-value coproducts has been recognized as the strategy to make biorefineries economical. Cull potato and crude glycerol from biodiesel industry were used in this research for the production of docosahexaenoic acid (DHA, 22:6, w-3) from the culture of marine algae Schizochytrium limacinum SR 21. This paper reports a two-stage process that significantly increased the algae concentration in the fermentor. The first stage is for cell number increase and second stage for cell size increase. The optimal culture conditions for the two stages were different. Based on this discovery, an innovative protocol was developed, in which the culture conditions of the two stages were optimized separately, and a “shifting” strategy was used. With is strategy, the cell culture density was significantly increased, and scale up work of this process showed great commercial potential of this process.

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