Sunday, April 29, 2007
2-42

Effect of different nutrient sources on the enzyme production by thermophilic fungus Thermoascus aurantiacus cbmai 756

Denise S. Oliveira, Eleni Gomes, Celia M. L. Franco, and Roberto Da Silva. Laboratorio de Bioquimica e Microbiologia Aplicada, IBILCE – Instituto de Biociencias Letras e Ciencias Exatas, UNESP, Rua Cristovao Colombo, 2265, Sao Jose do Rio Preto, Brazil

In recent years, the baking industry has focused its attention on the replacement of several chemical compounds by enzymes. Enzymes that hydrolyze non-starch polysaccharides as xylanases leads to the improvement of rheological properties of dough, bread specific volume and crumb firmness. The purpose of this work was to study the production of the xylanase and others enzymes involved with bread quality from a Brazilian  strain  isolated  of  Thermoascus  aurantiacus  on solid  state fermentation  using different substrates with saline solution (0,01% NH4 NO3, 0,01% NH4H2PO4 and 0,01% MgSO4). After 5 days of incubation the filtrate was used for assays of enzyme activities.

Table1. Effect of different substrates on enzyme production (U/ml).

Nutrient sources

Xylanase

Protease

a-Amylase

Glucoamylase

Wheat bran

100

141

9

<1

Corncob

179

33

10

<1

Corn straw

86

70

11

<1

Paper

11

79

10

<1

The fungus produced xylanase, protease and α-amylase independent of the material used as nutrient source. Glucoamylase production was insignificant in all substrates. Xylanase production from corncob was higher than wheat bran. Corncob was lower in protease production. This property is important because the protease can degrade the gluten protein.

 

Acknowledgements: The authors are grateful to CAPES for scholarship.