Sunday, April 29, 2007
2-42
Effect of different nutrient sources on the enzyme production by thermophilic fungus Thermoascus aurantiacus cbmai 756
In recent years, the baking industry
has focused its attention on the replacement of several chemical compounds by
enzymes. Enzymes that hydrolyze non-starch polysaccharides as xylanases leads to the improvement of rheological
properties of dough, bread specific volume and crumb firmness. The purpose of
this work was to study the production of the xylanase and others enzymes
involved with bread quality from a Brazilian
strain isolated of Thermoascus
aurantiacus on solid state fermentation using different substrates with saline
solution (0,01% NH4 NO3, 0,01% NH4H2PO4
and 0,01% MgSO4). After 5 days of incubation the filtrate was used
for assays of enzyme activities.
Table1. Effect of
different substrates on enzyme production (U/ml).
Nutrient sources | Xylanase | Protease | a-Amylase | Glucoamylase |
Wheat bran | 100 | 141 | 9 | <1 |
Corncob | 179 | 33 | 10 | <1 |
Corn straw | 86 | 70 | 11 | <1 |
Paper | 11 | 79 | 10 | <1 |
The fungus produced xylanase,
protease and α-amylase
independent of the material used as nutrient source. Glucoamylase
production was insignificant in all substrates. Xylanase
production from corncob was higher than wheat bran. Corncob was lower in
protease production. This property is important because the protease can degrade
the gluten protein.
Acknowledgements: The authors are grateful to CAPES for scholarship.
See more of General Submissions
See more of The 29th Symposium on Biotechnology for Fuels and Chemicals (April 29 - May 2, 2007)