Screening of cellulase- and hemicellulose- producing fungi was carried out from soil in Japan. We have isolated a promising fungal strain from soil in Okutama, Tokyo, which is especially excellent for xylan degradation. This strain (KIF78) was identified as an Aspergillus species according to the DNA sequencing of its ITS1 region. When culture broth of this fungus was used with Acremonium cellulase for the saccharification of ball-milled eucalyptus, higher xylose recovery was obtained than the digestion with Acremonium cellulase alone. Similar results were obtained for mannose recovery when pretreated Douglas fir was used as the substrate. These results show the efficacy of strain KIF78 for efficient saccharification of hemicellulose.