Sunday, April 29, 2007
2-28

Laccase production by basidiomycete strains on lignocellulosic agricultural wastes

Gomes, E. Gomes1, Carvalho, C. Carvalho1, and R. Silva2. (1) Biology, São Paulo State University-UNESP/IBILCE, R: Cristovao Colombo, 2265, Jd Nazareth, São José do Rio Preto, 15054-000, Brazil, (2) Chemistry and Environmental Science, São Paulo State University-UNESP/IBILCE, R: Cristovao Colombo, 2265, Jd Nazareth, São José do Rio Preto, 15054-000, Brazil

Laccase is an enzyme associated with the lignin-degrading ability of white rot fungi. Wood rotting Basidiomycetes collected at the “Estação Ecológica do Noroeste Paulista”, São José do Rio Preto, São Paulo, Brasil and identified as Coriolopsis byrsina 16, Lentinus sp 48, Phellinus rimosus 47 and Gloeophyllum striatum 40, produced maximum laccase activities of 3,000, 1,300, 550 and 300 U/ml, respectively, when cultured on wheat bran by solid state fermentation. Laccase activities from P. rimosus and C. byrsina were promoted by addition of nitrogen at concentrations up to 6 and 3 mg/g, respectively, while G. striatum was indifferent to N concentration up to 3mg/g, with a slight increase when 6mg/g was used. For all four species laccase activity was suppressed by 21mg/g of nitrogen. Medium added (NH4)2HPO4 afforded higher laccase production than with other N sources. The enzymes produced showed optimum pH at 3.0-3.5 and were stable at neutral pH values. C. byrsina 16, Lentinus sp 48 and G. striatum 40 produced laccase with optimal activities at 60-65 °C.

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