Monday, April 30, 2007
3-23

Cursory application of near infrared spectroscopy on anatomical fractions of barley (Hordeum vulgare) for component comparisons

Peter A. Pryfogle1, Christopher T. Wright2, Daniel M. Stevens3, and J. Richard Hess2. (1) Biological Sciences, Idaho National Laboratory - Battelle Energy Alliance, 2525 North Freemont Avenue, IRC - 603/Mail Stop 2203, Idaho Falls, ID 83415, (2) Renewable Energy & Power, Idaho National Laboratory - Battelle Energy Alliance, 2525 North Freemont Avenue, IRC - 603/Mail Stop 2203, Idaho Falls, ID 83415, (3) BYU - Idaho, Idaho National Laboratory - Battelle Energy Alliance, 2525 North Freemont Avenue, IRC - 603/Mail Stop 2203, Idaho Falls, ID 83415

Whole, mature Harrington barley plants were separated by hand into distinct anatomical fractions (i.e, node, internodes, leaves).  These fractions were further segregated according to their location along the plant stem from bottom to top node, internode, leaf, and grain head regions.  Baled barley feedstocks were preprocessed by grinding and separated according to their particle sizes and densities.  The anatomical and grind fractions were dried, ground to pass through a 2mm screen, and analyzed by NIR spectroscopy (Foss 6500).  Principal component analysis (PCA) was used to compare the collected spectra between anatomical and grind fractions.  Results showed that anatomical fractions could be separated into distinct PCA spaced groups.  There were similar distributions for the grind fractions.  Chemical analyses of the fractions were completed to determine the quality of the material with respect to potential ethanol yields.  It was possible to determine relative anatomical composition of grind fractions by comparing collected NIR spectra through statistical analysis.  This information may lead to the development of more efficient preprocessing methods that take advantage of relationship between plant anatomical and grind fractions to optimize lignocellulosic preprocessing requirements for reduced supply system costs and increased ethanol production.

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