The sucrose conversion was attained when carried out under initial substrate (20 to 100 mM) and invertase (3.0 to 24 U/mL), glucose oxidase (0.25 to 40 U/mL) and catalase (100 to 800 U/mL) concentrations in a membrane reactor operated in a fixed dilution rate (1.0 to 6.0 h^-1), temperature (30°C to 50°C) and pH 5.5. The optimized operational conditions led to a conversion yield of 100% for sucrose hydrolysis and glucose oxidation when utilizing 50 mM of sucrose and resulting in enzymes productivities of 4.2 mmol/U.h, 0.60 mmol/U.h and 0.00062 mmol/U.h, respectively, to invertase, glucose oxidase and catalase. In regard to the glucose oxidation, the addition of catalase in the reaction medium was necessary, in order to minimize the inhibition of the GO by the hydrogen peroxide formed.