Sunday, April 29, 2007

Participation of cytochrome p450 in biodegradation of aliphatic hydrocarbons in Mucor circinelloides a potential biorremediator

Roberto Zazueta-Sandoval, V. Odemaris Vallejo-Tinoco, Arelí Durón-Castellanos, and Hortencia Silva-Jiménez. Institute of Research in Experimental Biology, University of Guanajuato, Noria Alta s/n, Postal Code 36050, Guanajuato, Mexico

Cytochrome P450 monooxygenases found in microorganisms as well as plants and animals carry out many types of chemical transformations, including aliphatic hydroxilations, epoxidations, dealkylations, dehalogenations and various mechanism-based inactivations. In this work we describe the identification of Cytochrome P450 in strain YR-1 a filamentous fungus isolated from petroleum-contaminate soils, by means of immunodetection techniques using a commercial heterologous antibody against Cit P-450.

Initial studies indicate that there are different cit P450 in the strain depending of the carbon source used as sole carbon source for growth. In glucose, there are only one possible protein band meanwhile in aliphatic hydrocarbons (n-decane or n-hexadecane) was possible to detect two protein bands. Also we have several results from iso-picnic sucrose gradients that suggest the localization of cyt P450 in some kind of micro bodies different to peroxisomes.