Sunday, April 29, 2007

Peroxisome and peroxidase in biodegradation of hydrocarbons in yr-1 strain of Mucor circinelloides

Roberto Zazueta-Sandoval Sr., Hortencia Silva-Jiménez, and Arelí Durón-Castellanos. Institute of Research in Experimental Biology, University of Guanajuato, Noria Alta s/n, Postal Code 36050, Guanajuato, Mexico

Eukaryotic cells are characterized by the compartmentalization of various metabolic functions into separate subcellular organelles. In the case of aliphatic hydrocarbon biodegradation pathway, there are some reports in literature that localize the fatty alcohol oxidase that’s the second enzyme of the pathway in the peroxisomes. In previous reports of our own group working with a strain of a filamentous fungus, isolated from petroleum contaminated soils named YR-1, the results suggest that in the case of these strain, the fatty alcohol oxidase is located in a particular microbodies different to the peroxisomes. In this work we describe the identification by zymograms, the measure of enzymatic activity of the peroxidase and some molecular characteristics of it.