Sunday, April 29, 2007
1B-10

Omega hydroxylation of oleic acid using Bacillus pumillus

Wei Yan1, Zu-Fang Wu2, and Rakesh K. Bajpai1. (1) Chemical Engineering, University of Missouri - Columbia, W2030 Lafferre Hall, Columbia, MO 65211, (2) Faculty of Life Science and Biotechnology, Ningbo University, Ningbo, 315211, China

Treatment of Bacillus pumilus BD-174 with NTG (N-methyl-N’-nitro-N-nitrosoguanidine) and positive screening resulted in several mutants possessing improved capability to carry out selective hydroxylations at w-1 carbon of oleic acid.  One mutant,  named Bacillus pumilus MF641 (BP M-F641), was found to be an over producer of 17-hydroxy-9-octadecenoic acid from the parent fatty acid. Optimization of transformation conditions resulted in production of 797-mg/L 17-hydroxy-9-octadecenoic acid in shake-flask cultures.  The optimum conditions were 24-hour-old culture with media at pH 7.2 and 0.2g/L MnSO4, and incubation with oleic acid for four days at 300C. The highest conversion and yield were 15.86% and 27.01%, respectively.

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